Since its foundation, FLIM LABS has pursued a clear mission: making Fluorescence Lifetime Imaging Microscopy (FLIM) more accessible, intuitive, and affordable for every laboratory.
Through innovative hardware solutions and user-friendly phasor-based analysis tools, the company continues to simplify workflows and lower the barriers that have traditionally limited the adoption of FLIM technology.
However, democratizing FLIM is not only about simplifying data acquisition and analysis. Reliable and reproducible measurements require another fundamental element: calibration.
Calibration is an essential step in every FLIM experiment. It ensures the accuracy of lifetime measurements, validates instrument performance, and allows researchers to compare results across experiments, systems, and laboratories. Yet, suitable calibration standards are often difficult to access, prepare, or reproduce consistently.
To address this challenge, FLIM LABS has developed the FLIM-Phasors Calibration Kit, the company’s first solid calibration standard specifically designed for FLIM systems.
The kit includes a fluorescent lifetime reference sample featuring a well-defined monoexponential fluorescence decay with a lifetime of 2.5 ns, compatible with both 405 nm and 488 nm excitation wavelengths. This reference allows users to verify the Instrument Response Function (IRF), assess lifetime accuracy, and monitor instrument stability before performing experimental measurements.
In addition, the kit contains a Convallaria majalis biological reference sample, enabling qualitative evaluation of image quality and phasor plot positioning. Together, these two standards provide a practical solution for system validation, performance benchmarking, training activities, and method development.
The launch of this calibration kit represents another step toward FLIM LABS’ vision of making advanced fluorescence lifetime imaging available to a broader scientific community. By simplifying calibration and standardization, researchers can focus more on generating meaningful biological insights and less on technical complexity.
And this is only the beginning. Additional calibration standards optimized for other excitation wavelengths and applications are already under development, further expanding the FLIM LABS ecosystem and supporting the growing FLIM community worldwide.
